There are still many knowledge gaps in the biology of Anairetes reguloides. Much of the species' basic ecology still needs investigation. Little is known about the species’ reproductive investment and timeline, social behavior (both intra and interspecific), and habitat requirements. The Pied-crested Tit-Tyrant occurs in areas of partial cultivation, but Jaramillo (2003) reports that the species is absent in areas of heavy cultivation. At what point is the land degraded beyond use for Pied-crested Tit-Tyrant? Because of its intermediate level of sensitivity, the Pied-crested Tit-Tyrant could be a potential indicator species for the ecological health of human-dominated forest and scrub habitats of the western Andean slope.
Finally, additional studies are needed on the systematics of the A. reguloides and A. nigrocristatus species complex. Key gaps in knowledge include the phylogenetic relationships, appropriate species limits, and whether latitudinal geographic variation is clinal or discrete. Surveys are needed to address the status and distribution of Pied-crested Tit-Tyrant on the east slope of the Andes in southern Peru (Cusco and Apurimac), and potential connectivity with west slope populations.