The vocalizations of Spectacled Tyrannulet are not well characterized compared to other congeners. Call in the Andes are described as "2-5 sad, halting, slightly descending whistles, each note fractionally lower than previous, wheeeeaa,. . . . wheeeeaa, .... etc., notes often hurried at [the] end" (Hilty 2003). Geographic variation in vocalizations within this species has not been described, but perhaps may be expected (see Geographic Variation).
Additional audio recordings of vocalizations of Spectacled Tyrannulet can be heard at xeno-canto.