Alvarez and Whitney (2001) describe three vocalizations of Mishana Tyrannulet, and to date recordings of the Nanay and Tigre river basins population have not revealed any additional vocalization types. By far the most commonly heard (and the most commonly recorded) vocalization is a daytime call consisting of 1-3 (usually two) short rising whistles in the 4-5.5 kHz band. This vocalization occasionally is delivered with a slight burry quality. The dawn song consists of 2-4 similar notes that differ from those of the day call in that they begin flatter and rise more abruptly towards the end of each syllable. Alvarez and Whitney (2001) also report a more complex multi-note call that is seldom heard and consists of a descending, accelerating series of whistled notes near 4 kHz.
The commonly heard vocalizations of the Mayo Valley population are similar to the daytime call of the Loreto form, but begin with a much stronger initial downward component. An additional vocalization has been recorded from the Mayo Valley and has been attributed to this species; this vocalization consists of a slightly descending series of seven sharply descending lisping notes.