The song is a tremulous whistle, given singly or in series. Descriptions of the song include: "single whistles, and the 1st of a series, tend to swell then fade, successive whistles rise slightly, and a series often intensifies overall, ending abruptly: wheeee-eeer, wheeeeer, wheeeeer..., etc." (Howell and Webb 1995); "There are two main calls, one a series of clear tremulous whistles, each higher in pitch than the preceding, increasing in volume and rapidity and ending abruptly; the other ... sliding up about a half tone, then down about a full tone" (Ridgely and Gwynne 1989); and "song, crespuscular, a series of quavering whistles rising in pitch and accelerating in pace. Daytime call a single quavering note rising and then quickly dropping in pitch: heeEE'E'u'u'u'u'u'u" (Lane, in Schulenberg et al. 2007).
The vocalizations of Little Tinamou are similar to those of Great Tinamou (Tinamus major), but are higher pitched and less resonant.