Amazonian Pygmy-Owl is widely distributed and is uncommon to fairly common across its range. Nonetheless, most aspects of its natural history are very poorly known. In large part, this is because the species occupies the upper strata of tall forest, and so is not easily observed. There is very little information on its breeding biology or on its diet. There also is little or no information on such topics as age at first breeding, life span and survivorship, dispersal, population regulation, and territoriality for Amazonian Pygmy-Owl.